Bio X4 Reviews: Does It Really Work? All Facts 2024


Reviewed by Jocelyn Chen, BME
bio x4 reviews
Bio X4 includes probiotics for a healthy digestive tract. Photo: Team Design

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As the demand for nutritional supplement products supporting weight loss grows, so has the number of products to choose from. Bio X4, provided by Nucific, offers a product that may check multiple boxes for many people. It is designed to support both weight management and healthy digestion.

The Nucific brand was established in 2014 under the guidance of Dr. Amy Lee, a board-certified physician specializing in obesity management. The brand has been intended to help more people reach optimal health and has built a significant following in recent years.

Over-the-counter dietary supplements offering benefits for weight loss and gut health tend to combine various ingredients. Examining each ingredient in more detail can help explore whether these products will likely be safe and effective. We will also consider the quality of the manufacturing process and feedback from real Nucific bio-x4 4-in-1 weight management probiotic supplement reviews.

So, how does Bio-X4 stack up? 

Bio X4

Bio X4

  • GMP-certified manufacturing.
  • Third-party testing.
  • Beneficial probiotic blend.
  • Price, $99 on the manufacturer’s website without a subscription.
  • Proprietary blends.
  • Some research is mixed.

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Nucific Bio X4 Reviews


Over-the-counter supplements can vary widely in terms of quality, so it is helpful to look for signs of dependable manufacturing practices. Here are a few signs that Bio X4 and Nucific provide high-quality products:

  • Manufactured in a Good Manufacturing Practices or GMP-certified facility.
  • No history of FDA warning letters.
  • Independent third-party testing.
  • Better Business Bureau accreditation with an A+ rating.

GMP certification is often included on the label of products as a sign of quality. Certification means the facilities that produce the supplement are maintained to FDA standards related to cleanliness, equipment, ingredient storage, and staff training.

It is important to note that the FDA does not evaluate over-the-counter products directly, so these standards apply only to the facility itself. By consistently upholding these standards, manufacturers can avoid being issued a warning letter by the FDA.

Independent third-party testing provides a means of ensuring the correct ingredients are provided at the correct dosages. These testing procedures also confirm that the product is free of potentially harmful contaminants. Unfortunately, there are reports[1] of many over-the-counter products containing ingredients with the potential to cause toxicity. 

Independent third-party testing and GMP certification help promote transparency within the manufacturing process. These are important signs that a product is more likely to provide consistent quality.

So, Bio X4 will likely provide consistent ingredients, but which ingredients are included?

Bio X4 Ingredient Analysis

The active ingredients included in Bio-X4 are broken down into four categories meant to support the four primary claims of benefit. These ingredients include:

bio x4 reviews
Bio X4 Ingredients
  • Probiotic Blend, 4 billion colony-forming units, or CFU per capsule: 
    • Lactobacillus blend:
      • Lactobacillus acidophilus.
      • Lactobacillus rhamnosus.
      • Lactobacillus plantarum.
  • Bifidobacterium blend:
    • Bifidobacterium lactis. 
    • Bifidobacterium animalis. 

Lactobacillus and bifidobacterium strains are commonly used probiotic supplements meant to support overall digestive health. 

  • Digestive Enzyme Blend, 43 mg total:
    • Amylase.
    • Bromelain.
    • Lipase.

Amylase, bromelain, and lipase are digestive enzymes meant to offer additional support for digestive health. 

  • Weight Management Blend, 100 mg:
    • Green Tea Extract, listed as 50% EGCG or epigallocatechin gallate. 

Green Tea Extract is an herbal supplement that provides important individual components. Green tea is a source of caffeine, although extracts tend to vary in potency.[2] Assuming there may be as much as 10% caffeine by weight, there may only be as much as 10 mg in the supplement.

Green tea extract also provides catechins like EGCG. Catechins have been associated with various potential health benefits.[3] Overall, green tea extract is included in Bio X4 to help promote weight management and boost metabolism.

  • Craving Control Blend, 166 mg:
    • Slimaluma (Caralluma fimbriata).

Caralluna fimbriata, the active ingredient in Slimaluma, is an herbal supplement[4] derived from a shrub with cactus-like leaves. It is meant to provide appetite suppression.

The inactive ingredients are standard components of an over-the-counter supplement. These are meant to help form the capsule and ensure the ingredients are uniformly mixed:

  • Vegetable cellulose. 
  • Microcrystalline cellulose. 
  • Stearic Acid. 
  • Silica. 

These ingredients are derived from natural sources and are unlikely to cause allergic reactions.

So, what does clinical research tell us about these ingredients and their potential risks and benefits?

Support Research

Manages “junk” cravingsModerate Evidence
Promotes weight managementModerate Evidence
Supports smoother digestionConcrete Evidence
Boosts metabolismModerate Evidence

Manages “Junk” Cravings

Caralluma fimbriata extract has been evaluated for use as a potential appetite suppressant. It has been associated with benefits for children with Prader-Willis Syndrome (PWS), a rare genetic disorder associated with a constant sense of hunger. The effects in other populations have been less clear.

Successful weight loss has been shown in a small trial[5] enrolling overweight individuals who do not have PWS. A subsequent larger review[6] of multiple studies suggests that there may be no benefit, however.  

Promotes Weight Management

Green tea extract and EGCG are the primary ingredients that are meant to aid weight loss. Green tea does contain caffeine,[7] which may aid in weight loss efforts. It is unlikely that there is enough caffeine in Bio X4 to provide significant support, however.

EGCG is considered the most potent catechin provided by green tea, which is likely why Bio X4 highlights the concentration.

Studies[8] have been conducted related to green tea and EGCG’s ability to help individuals lose weight. These studies featured a fairly wide range of doses, and results were mixed. Some patients did experience a reduction in body mass index, with effects generally more pronounced in overweight individuals. Eating a healthy diet and staying hydrated will provide additional support for your weight loss efforts. 

Supports Smoother Digestion

Digestive enzymes with established benefits like amylase and lipase[9] are included in the formulation, but are provided as a part of a proprietary blend. These blends make it difficult to assess whether the dosage of individual ingredients provided is likely to be beneficial. It is also important to acknowledge that the established benefit has been shown for individuals managing conditions like pancreatic insufficiency or diabetes.

The best evidence for digestive support is seen in probiotics. Some of these benefits include:

Probiotics have also been linked to a number of other potential benefits.[13]

Boosts Metabolism

Metabolism is often measured by monitoring blood glucose control, cholesterol levels, body weight, and blood pressure. A variety of studies[14] have been conducted to observe the effects of green tea and its primary catechin EGCG.

Results were mixed when considering each area aside from blood glucose, with benefits seen more specifically in individuals managing diabetes. There is a potential for benefit in these areas, but additional studies would be helpful to provide a clearer picture.

Low levels of enzymes like amylase[15] have been associated with obesity and diabetes, so the case could be made that supplementation may be beneficial in healthy adults. However, there are no studies supporting a clear benefit for the prevention of metabolic disorders.

Bio X4 Reputation

As with any over-the-counter nutritional supplement, the official website comes packed with rave reviews handpicked by the manufacturer. Nucific does have an official listing on Amazon, and the reviews provided there may provide a more complete picture of individual experiences. Overall the Nucific Bio X4 reviews on Amazon are generally positive, and Bio X4 has a rating of 3.8 out of 5.

Some of the reviews are very positive and highlight personal experiences with weight loss and improvements in digestive symptoms. The negative reviews focus on a lack of any beneficial effect, gastrointestinal side effects, and concerns with the return policy. It is worth noting that buying directly from the official website may result in an improved experience if you are trying to utilize the return policy.

Bio X4 Price

The price of Bio X4 on the official website is quite high at $99 for what will provide either a 15 or 30-day supply, depending on your chosen dosage. The price is cut in half to $49 if you choose to become a member. On Amazon it is available for about $50, but reviews do mention concerns about the return policy when purchased on the platform.

Alternatives To Bio X4

Total Restore

Total Restore
  • GMP-certified manufacturing.
  • Third-party tested. 
  • Natural ingredients.
  • High price, $70.
  • Contains shellfish.

Colon Broom

colon broom
  • GMP-certified manufacturing.
  • Third-party tested.
  • Flavor options.
  • Psyllium fiber only.
  • High price, $63.

Gundry MD Bio Complete 3

  • GMP-certified manufacturing.
  • Third-party tested. 
  • 90-day guarantee.
  • High price, $70.

How Bio X4 Works

Bio X4 provides a variety of ingredients providing support in multiple ways. The strongest research points to a higher likelihood of benefit for some individuals: 

  • Bio X4 may be particularly helpful for individuals with digestive concerns as its probiotic strains promote healthier gut bacteria. 
  • Green tea and EGCG may be more likely to promote healthy metabolism for individuals with an existing metabolic disorder like diabetes
  • Weight loss benefits may be more likely for individuals who are overweight or obese

It is always best to consult your doctor prior to starting an over-the-counter supplement. This will help you ensure the product will be safe and effective for your individual needs. 

Health Benefits Of Bio X4

In addition to the effects directly mentioned by the manufacturer, the ingredients of Bio X4 have been studied for other purposes. 

Green tea[3] has been associated with numerous potential health benefits, including: 

  • Cancer prevention.
  • Liver protection (animal models).
  • Improved retention of memory (animal models). 
  • Anti-inflammatory effects. 
  • Improved immune response. 

Probiotics[13] may provide beneficial effects for stress management, including anxiety and depression. Research is continuing to develop in this area. 

Potential Side Effects

The side effects mentioned in research align with those reported in individual reviews of Bio X4. These are primarily gastrointestinal effects, including: 

  • Upset stomach.
  • Gas and bloating
  • Diarrhea.
  • Constipation.

It is possible that symptoms may subside after an adjustment period of a few days. It is also important to keep in mind that diet can impact these symptoms as well.

How To Take Bio X4

The official website and label for Bio X4 recommend using one capsule three times daily with meals. A maximum dose of up to two capsules three times daily, or six total, is also mentioned. If you miss a dose, start back up at the next scheduled time. Do not take a double dose.

Bio X4 Customer Reviews – What Do Real Users Say?

I didn’t really notice a lot of difference with this product and others like it. You do get a lot but then you have to use it three times a day. Other products are cheaper and help digestion just as well. No discernible weight loss.

– Kindle Customer

I have all types of food sensitivities, and this was the one product that got me back to a solid bowel movement. I can’t say it helped my weight at all but at least I’m not having the diarrhea issues I one had. It’s expensive but it works better than any digestive enzymes I have tried. No more panic runs to the bathroom.

– Gregory B.


Bio X4 is relatively expensive, but does provide a product with reliable quality. Some individuals may be more likely to benefit from using the supplement than others. Prior to starting a supplement, it is best to consult your doctor to ensure the product will be safe and effective for your individual needs. 

Frequently Asked Questions

Is Bio X4 legitimate?

Yes, it is produced in a GMP-certified facility, has had no warning letters issued by the FDA, and undergoes third-party testing. The results may vary between individuals.

Can I take Bio X4 without food?

Bio X4 is more likely to be effective when taken with meals. Digestive enzymes need something to digest, and probiotics are more likely to survive the trip to the large intestine when taken with meals.

Can you drink alcohol with Bio X4?

Direct interactions with small amounts of alcohol are unlikely, but drinking may not be beneficial for digestive symptoms or weight loss efforts.

Can I take probiotics for a long time?

Yes, probiotics can be taken daily over the long term. Individuals over the age of 65 should consult their doctor prior to considering use.

How long does it take for probiotics to work?

In some instances beneficial effects are seen within days, but results do vary among individuals.

+ 15 Sources

EHproject has strict sourcing guidelines and relies on peer-reviewed studies, academic research institutions, and medical associations. We work mostly with peer-reviewed studies to ensure accurate information. We avoid using tertiary references. You can learn more about how we ensure our content is accurate and current by reading our editorial policy.

  1. Tucker, J., Fischer, T., Upjohn, L., Mazzera, D.M. and Kumar, M. (2018). Unapproved Pharmaceutical Ingredients Included in Dietary Supplements Associated With US Food and Drug Administration Warnings. JAMA network open, [online] 1(6), pp.e183337–e183337. doi:
  2. Sabu Mandumpal Chacko, Thambi, P.T., Ramadasan Kuttan and Ikuo Nishigaki (2010). Beneficial effects of green tea: A literature review. Chinese Medicine, [online] 5(1), pp.13–13. doi:
  3. Mani Iyer Prasanth, Bhagavathi Sundaram Sivamaruthi, Chaiyavat Chaiyasut and Tewin Tencomnao (2019). A Review of the Role of Green Tea (Camellia sinensis) in Antiphotoaging, Stress Resistance, Neuroprotection, and Autophagy. Nutrients, [online] 11(2), pp.474–474. doi:
  4. Anwar, R., Roshina Rabail, Allah Rakha, Marcin Bryła, Roszko, M., Rana Muhammad Aadil and Marek Kieliszek (2022). Delving the Role of Caralluma fimbriata: An Edible Wild Plant to Mitigate the Biomarkers of Metabolic Syndrome. Oxidative Medicine and Cellular Longevity, [online] 2022, pp.1–17. doi:
  5. Astell, K.J., Mathai, M.L., McAinch, A.J., Stathis, C.G. and Su, X. (2013). A pilot study investigating the effect of Caralluma fimbriata extract on the risk factors of metabolic syndrome in overweight and obese subjects: a randomised controlled clinical trial. Complementary Therapies in Medicine, [online] 21(3), pp.180–189. doi:
  6. Ranil Jayawardena, Francis, T.V., Sachith Abhayaratna and Ranasinghe, P. (2021). The use of Caralluma fimbriata as an appetite suppressant and weight loss supplement: a systematic review and meta-analysis of clinical trials. BMC Complementary Medicine and Therapies, [online] 21(1). doi:
  7. The effects of caffeine intake on weight loss: a systematic review and dos-response meta-analysis of randomized controlled trials. (2019). Critical Reviews in Food Science and Nutrition. [online] doi:
  8. Elahe Esmaeilpanah, Bibi Marjan Razavi and Hosseinzadeh, H. (2021). Green tea and metabolic syndrome: A 10-year research update review. DOAJ (DOAJ: Directory of Open Access Journals), [online] 24(9), pp.1159–1172. doi:
  9. Ianiro, G., Pecere, S., Giorgio, V., Gasbarrini, A. and Cammarota, G. (2016). Digestive Enzyme Supplementation in Gastrointestinal Diseases. Current Drug Metabolism, [online] 17(2), pp.187–193. doi:
  10. Shen, J., Zuo, Z. and Mao, A. (2014). Effect of Probiotics on Inducing Remission and Maintaining Therapy in Ulcerative Colitis, Crohnʼs Disease, and Pouchitis. Inflammatory Bowel Diseases, [online] 20(1), pp.21–35. doi:
  11. Pattani, R., Palda, V.A., Hwang, S.W. and Shah, P.S. (2013). Probiotics for the prevention of antibiotic-associated diarrhea and Clostridium difficile infection among hospitalized patients: systematic review and meta-analysis. Open medicine : a peer-reviewed, independent, open-access journal, [online] 7(2), pp.e56-67. Available at: [Accessed 14 Nov. 2023].
  12. Zhang, Y., Li, L., Guo, C., Mu, D., Feng, B., Zuo, X. and Li, Y. (2016). Effects of probiotic type, dose and treatment duration on irritable bowel syndrome diagnosed by Rome III criteria: a meta-analysis. BMC Gastroenterology, [online] 16(1). doi:
  13. Vadim Osadchiy, Martin, C.R. and Mayer, E.A. (2019). The Gut–Brain Axis and the Microbiome: Mechanisms and Clinical Implications. Clinical Gastroenterology and Hepatology, [online] 17(2), pp.322–332. doi:
  14. Elahe Esmaeilpanah, Bibi Marjan Razavi and Hosseinzadeh, H. (2021). Green tea and metabolic syndrome: A 10-year research update review. DOAJ (DOAJ: Directory of Open Access Journals), [online] 24(9), pp.1159–1172. doi:
  15. Nakajima, K. (2016). Low serum amylase and obesity, diabetes and metabolic syndrome: A novel interpretation. World Journal of Diabetes, [online] 7(6), pp.112–112. doi:


Matthew Sommers is a clinical pharmacist with more than 10 years of experience in the pharmacy profession. He has most recently transitioned from a leadership role in a community setting into clinical practice with a focus… See More